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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 424
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Cell_line_name |
1G1
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Brief_description |
confirmed as mouse with IEF of AST, LDH, NP and with species-PCR cell line was
established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse
immunized with enriched retinal cell membranes; described as secreting a mouse
monoclonal antibody (IgM) reacting with the chicken dorsal retina cytoplasmic
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Description |
mouse hybridoma (anti-chicken dorsal retina cytoplasmic membranes, E12) cell line
was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse
immunized with enriched retinal cell membranes; described as secreting a mouse
monoclonal antibody (IgM) reacting with the chicken dorsal retina cytoplasmic
membranes (E12) and binding outer photoreceptor elements of adult vertebrate retina
(inclusive chick, mouse, rat) confirmed as mouse with IEF of AST, LDH, NP and with
species-PCR
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Depositor |
Dr. B. Schlosshauer, Natural and Medical Science Institute (NMI), Reutlingen,
Germany
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Morphology |
spheroidal cells growing singly in suspension, or occasionally loosely adherent
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Culture_conditions |
90% RPMI 1640 + 10% FBS + HFCS ("Hybridoma Fusion and Cloning Supplement"
for the first 1-2 weeks post-thawing) maintain at ca. 0.1-0.3 x 106 cells/ml; split
saturated culture 1:3 to 1:10 every 2-5 days (some cells attach to the surface of the
culture flask, but can be detached by tapping the flask); seed out at ca. 0.3 x 106
cells/ml; a maximal density of 1.0 x 106 cells/ml should not be exceeded at 37C with
5% CO2 cell harvest of ca. 1.0 x 106 cells/ml
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Viruses |
ELISA: reverse transcriptase negative; PCR: SMRV -
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Properties |
secretion of a IgM monoclonal antibody
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule
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Doubling_time |
ca. 30 hours
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Mycoplasma |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Cytogenetics murine flat-moded hypertetraploid karyotype - 94-102<4n> - no centric fusion
markers present
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Bibliographic_reference |
Schlosshauer et al., Eur J Cell Biol, 73: 3 (1997) [PMID: 9208228
] |
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